Analyzing ligation mixtures using a PCR based method

A simple rapid detection method of DNA based on ligation-mediated real-time fluorescence PCR.

Polymerase chain reaction (PCR) has been widely used for detecting long chain DNA or RNA of viruses, bacteria and cytokines, but it is difficult to detect DNA or RNA with short length sequences. In this work, we developed a simple and rapid detection method for short length DNA sequences in complicated matrices based on ligation-mediated PCR. Two probes, both designed as 52 bases and respective...

Analysis of microRNA signatures using size-coded ligation-mediated PCR

The expression pattern and regulatory functions of microRNAs (miRNAs) are intensively investigated in various tissues, cell types and disorders. Differential miRNA expression signatures have been revealed in healthy and unhealthy tissues using high-throughput profiling methods. For further analyses of miRNA signatures in biological samples, we describe here a simple and efficient method to dete...

In vivo footprinting using UV light and ligation-mediated PCR.

Fast ligation-mediated PCR, a fast and reliable method for IS6110-based typing of Mycobacterium tuberculosis complex.

IS6110 restriction fragment length polymorphism (RFLP) analysis is the most widely applied method for strain differentiation of Mycobacterium tuberculosis complex. We have previously described mixed-linker PCR, an IS6110-based PCR method that favorably compared with other typing methods for M. tuberculosis complex according to reproducibility and ability to differentiate between strains. Here w...

PCR with End Trimming and Cassette Ligation: A Rapid Method to Clone

We described a method for PCR amplification of unknown flanking genomic DNA fragments. This method is a combination of PCR with "endtr imming method" and "cassettes and cassette-primers method". In this method, genomic DNA was digested with three different groups of restriction enzymes. DNA in group 1 was digested with BamHI, Bglll, Fbal, or Mbol. DNA in group 2 was digested with Blnl, Nhel, Sp...